Journal: Nature Communications
Article Title: Zika virus NS1 drives tunneling nanotube formation for mitochondrial transfer and stealth transmission in trophoblasts
doi: 10.1038/s41467-025-56927-2
Figure Lengend Snippet: A Rotenone is a reversible mitochondrial electron transport chain complex I inhibitor. B Cytotoxicity of rotenone in JEG-3, n = 4, 48 hrs. C Rotenone inhibits ZIKV growth in JEG-3 infected cells (MOI = 0.1, 48 hrs, n = 3) as determined by plaque assay. D Miro1 reducer induces proteasomal degradation of MIRO1. E Cytotoxicity of Miro1 reducer in JEG-3 cells, n = 4, 48 hrs. F Miro1 reducer inhibits ZIKV growth in JEG-3 infected cells (MOI = 0.1, 48 hrs, n = 3) as determined by plaque assay. G - K IFN levels in ZIKV infected (MOI = 0.1, 48 hrs, n = 4) JEG-3 cells treated with Rotenone (0.01 μM; DMSO 0.0001%) and Miro1 reducer (40 μM; DMSO 0.4%). Secreted IFN levels in ZIKV-infected cells treated with rotenone or Miro1 reducer are expressed in pg/mL. Data are presented as median and interquartile range (G, H) and mean ± SEM (B, C, E, F, I, J, K). Statistical significance was determined by ANOVA followed by Dunnett’s multiple comparison tests (B, C, E, F, I, J, K) and Kruskal-Wallis followed by Dunn’s multiple comparison tests (G, H). *= P ≤ 0.05, ***= P ≤ 0.001, and ****= P ≤ 0.0001. Figures A and D were created in BioRender.129. Source data are provided as a Source Data file.
Article Snippet: ZIKV-infected trophoblast cells were treated with either Rotenone (Sigma, #R8875), an inhibitor of mitochondrial respiratory chain complex I, or Miro1 Reducer (Tocris Bioscience, #7091), a compound that promotes the degradation of RHOT1/Miro1 (PMID: 31564441).
Techniques: Infection, Plaque Assay, Comparison